Dissection of organizer and animal pole explants from Xenopus laevis embryos and assembly of a cell adhesion assay.

Fibronectin, 0.1% solution Reagent Sigma-Aldrich F-1141 This pre-made solution can be used as 50x stock. Make 1/50 dilution with 1x MBS-H make 20ug/ ml working solution just before you use. You need 200ul per samples of this working solution.I do NOT recommend using solid Fibronectin, because it is difficult to dissolve at 0.1% concentration to make the stock solution. Although it is more expensive, this pre-made 0.1% solution is better to obtain consistent results.